Isolation and purification of extracellular phytase from Sporobolomyces japonicas (L9)
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چکیده
منابع مشابه
The structure of extracellular phosphorylated galactans from Sporobolomyces yeasts.
Two strains of SporoboZomyces pecies, NRRL Y-6493 and NRRL Y-6502, produce extracellular, lightly acetylated, phosphorylated galactans when grown on media containing glucose as the carbon source. D-&lactose and D-galactose 6-phosphate were the only carbohydrate components present in strong acid hydrolysates. oc-D-Galactose 1 -phosphate was isolated after vigorous alkaline hydrolysis. Periodate ...
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Bacillus species producing a thermostable phytase was isolated from soil, boiled rice, and mezu (Korean traditinal koji). The activity of phytase increased markedly at the late stationary phase. An extracellular phytase from Bacillus sp. KHU-10 was purified to homogeneity by acetone precipitation and DEAE-Sepharose and phenyl-Sepharose column chromatographies. Its molecular weight was estimated...
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An extracellular phytase from Aspergillus niger 11T53A9 was purified about 51-fold to apparent homogeneity with a recovery of 20.3% referred to the phytase activity in the crude extract. Purification was achieved by ammonium sulphate precipitation, ion chromataography and gel filtration. The purified enzyme behaved as a monomeric protein with a molecular mass of about 85 kDa and exhibited maxim...
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Fifty isolates capable of producing phytase were isolated from various soil samples. Out of fifty isolates five isolates showed maximum phytase activity. P6 isolate was selected for further study P6 isolate was biochemically characterized as Pseudomonas spp. The culture conditions were optimized for maximum enzyme production. The best carbon and nitrogen sources for maximum phytase production w...
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Background: Cholesterol oxidase (CHO) has various clinical and industrial applications. Recently, microbial CHO have received a great attention for their wide usage in medicine. Here, taxonomic characterizations of isolated strain from soil, optimization of the conditions for CHO production and biochemical characterizations of produced CHO enzyme were described. Finally, CHO gene was cloned int...
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ژورنال
عنوان ژورنال: TAP CHI SINH HOC
سال: 2012
ISSN: 0866-7160,0866-7160
DOI: 10.15625/0866-7160/v34n3se.1779